Studies Involving a Commercial Extract of Three Ballerina: ii- Evaluation of the in Vitro Effect on the Labeling of Blood Constituents of rats with Technetium-99m and on the Morphology of the Red Blood Cell.

The aim of this work is to characterize (electric conductivity and refractive index) a Three Ballerina (TB) and to evaluate its in vitro effect on the labeling of blood constituents with 99mTc and on the morphology of the red blood cells (RBC). Anticoagulated whole blood (Wistar rat) was incubated with a TB extract and the labeling of the blood constituents with technetium-99m (99mTc) was performed. Plasma (P) and blood cells (BC) were isolated and aliquots were also precipitated with trichloroacetic acid to separate soluble (SF) and insoluble fractions (IF). The %ATI in these samples was calculated. The morphology of the treated RBC showed no shape’s qualitative alterations. The TB extract was characterized with an electric conductivity of 1.35±0.04mSv/cm and refractive index of 2.21±0.15%BRIX. TB extract decreased significantly (p<0.05) the radioactivity distribution in the cellular compartment from 96.97±1.30% to 88.48±7.13%, and in IF-P from 74.29±4.12 to 14.26±5.73%. In conclusion, our data show some physical chemical parameters that could be suitable to characterize the preparation of an extract of TB. Moreover, substances present in the TB extract should probably have an effect on transport of the ions through the RBC membrane and/or should have redoxi properties and the stannous ion would decrease and could justify the effect on the fixation of the radioactivity on the plasma proteins. Moreover, although our experiments were carried out with animals, it is suggested precaution in the interpretation of the examinations that use labeled blood constituents in patients who are undergone TB extract.

Studies Involving a Commercial Extract of three Ballerina: I- Characterization and the Effect on the Binding of two 99mtechnetium-Radiopharmaceuticals on Blood Constituents.

We have determined some physicochemical parameters of an aqueous extract of Three ballerina (3TB) and its influence on the binding of the radiopharmaceuticals sodium pertechnetate (Na99mTcO4) and 99mTc-methylene diphosphonate (MDP-99mTc) on blood constituents. Whole blood of rats was incubated with a 3TB extract and with Na99mTcO4 or MDP-99mTc. Plasma (P) and blood cells (BC) from Wistar rats (control and treated) were separated. P and BC were precipitated with trichloroacetic acid (TCA) and soluble (SF) and insoluble fractions (IF) isolated. The percentage of incorporated radioactivity (%ATI) in each fraction was determined. The treatment not influenced statistically (p>0.05) the %ATI of both radiopharmaceuticals on BC and P compartments, as well as on IF-P and SF-P isolated by TCA precipitation. However, 3TB has altered the fixation of the 99mTc-MDP on the IF-BC and SF-BC. We are firstly reported physicochemical parameters (absorption spectrum, viscosimetry and phmetry) about an aqueous 3TB extract. Moreover, it is possible to speculate that an alteration would be not found on the biodistribution of the 99mTc-pertechnetate in the person that is undergoing 3TB extract. However, this would be found with the 99mTc-MDP.